Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India | Zendy

Aman Kamboj | Zendy; Atul Kumar Pateriya | Zendy; Anamika Mishra | Zendy; Pradip B. Ranaware | Zendy; Diwakar D. Kulkarni | Zendy; Ashwin Ashok Raut | Zendy

Open Access

Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India

Author(s) -

Aman Kamboj,

Atul Kumar Pateriya,

Anamika Mishra,

Pradip B. Ranaware,

Diwakar D. Kulkarni,

Ashwin Ashok Raut

Publication year - 2014

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2014/496219

Subject(s) - crimean–congo hemorrhagic fever , taqman , virology , real time polymerase chain reaction , zoonotic disease , medicine , transmission (telecommunications) , biology , disease , pathology , tick , gene , biochemistry , electrical engineering , engineering

Crimean-Congo hemorrhagic fever (CCHF) is an emerging zoonotic disease in India and requires immediate detection of infection both for preventing further transmission and for controlling the infection. The present study describes development, optimization, and evaluation of a novel molecular beacon-based real-time RT-PCR assay for rapid, sensitive, and specific diagnosis of Crimean-Congo hemorrhagic fever virus (CCHFV). The developed assay was found to be a better alternative to the reported TaqMan assay for routine diagnosis of CCHF.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research