Comparative Analysis of the Methanogen Diversity in Horse and Pony by UsingmcrAGene and Archaeal 16S rRNA Gene Clone Libraries | Zendy

Khin-Ohnmar Lwin | Zendy; Hiroki Matsui | Zendy

Open Access

Comparative Analysis of the Methanogen Diversity in Horse and Pony by UsingmcrAGene and Archaeal 16S rRNA Gene Clone Libraries

Author(s) -

Khin-Ohnmar Lwin,

Hiroki Matsui

Publication year - 2014

Publication title -

archaea

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.8

H-Index - 40

eISSN - 1472-3654

pISSN - 1472-3646

DOI - 10.1155/2014/483574

Subject(s) - pony , biology , methanogen , 16s ribosomal rna , population , library , ribosomal rna , alphaproteobacteria , genetics , gene , bacteria , demography , sociology

Comparative analysis of methanogen compositions in the feces of horse and pony was carried out by constructing the α -subunit of methyl coenzyme-M reductase ( mcrA ) gene and 16S ribosomal RNA gene (16S rRNA) clone libraries. The mcrA clone library analysis indicated that Methanomicrobiales was predominant in both horse and pony. Furthermore, most of the clones of the 16S rRNA gene library showed that Methanomicrobiales was also predominant in horse and pony, but the LIBSHUFF analysis showed that the horse and pony libraries were significantly different ( P < 0.05). Most of operational taxonomic units (OTUs) showed low similarity to the identified methanogens in both the mcrA and the 16S rRNA clone libraries. The results suggest that horse and pony harbor unidentified and novel methanogens in their hindgut. The methanogen population was higher in horse than in pony; however, the anaerobic fungal population was similar in horse and pony. The methanogen diversity was different between two breeds of Equus caballus .

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