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This work is carried out with  Gardenia coronaria  leaves that belong to the family Rubiaceae, which is a small-to-medium-sized but tall, deciduous tree, 7.6–9 m high on an average. Leaves are used for the treatment of rheumatic pain and bronchitis. The leaf of the plant consists of coronalolide, coronalolic acid, coronalolide methyl ester, ethyl coronalolate acetate triterpenes ( secocycloartanes ), and so forth. Methanol extract from the leaves of  Gardenia coronaria  was completely screened for membrane stability and antibacterial activity. The lower concentrations of Methanolic leaf extract of  Gardenia coronaria  gave good antimicrobial and anti-inflammatory activity, but higher concentrations gave relatively more projecting antibacterial activity  in vitro  as compared with Kanamycin. The crude drug's anti-inflammatory effects were compared with those of Aspirin as positive control. The Methanolic extracts of  Gardenia coronaria  leaves possessed a broad spectrum antibacterial activity against a variety of both Gram-negative and Gram-positive organisms like  Streptococcus agalactiae ,  Escherichia coli ,  Pseudomonas aeruginosa ,  Bacillus cereus ,  Shigella sonnei ,  Shigella boydii , and  Proteus mirabilis , with a zone of inhibition from 10 to 16 mm. The extract also showed good membrane stability to be considered as having significant anti-inflammatory action.

Antibacterial Activities andIn VitroAnti-Inflammatory (Membrane Stability) Properties of Methanolic Extracts ofGardenia coronariaLeaves