Antagonistic Evaluation of Bioactive Metabolite from Endophytic Fungus, Aspergillus flavipes KF671231 | Zendy

Ankita Verma | Zendy; B. N. Johri | Zendy; Anil Prakash | Zendy

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Antagonistic Evaluation of Bioactive Metabolite from Endophytic Fungus, Aspergillus flavipes KF671231

Author(s) -

Ankita Verma,

B. N. Johri,

Anil Prakash

Publication year - 2014

Publication title -

journal of mycology

Language(s) - English

Resource type - Journals

eISSN - 2356-7481

pISSN - 2314-6168

DOI - 10.1155/2014/371218

Subject(s) - sclerotinia sclerotiorum , plant use of endophytic fungi in defense , bioassay , biology , secondary metabolite , fungus , metabolite , botany , endophyte , aspergillus , fungal pathogen , pathogen , microbiology and biotechnology , biochemistry , genetics , gene

Of the total 40 endophytic fungi isolated from foliar tissues of medicinal plant Stevia rebaudiana Bertoni, a fungal isolate, Aspergillus flavipes, was subjected to bioassay guided fractionation. The fractionation was found active against medicinal plant pathogen Sclerotinia sclerotiorum with an inhibition zone of 29 mm in size. Further the metabolite was extracted which shows 20% growth inhibition in 24 h and 46% after 48 h, respectively. Bioassay guided chemical compound was identified as 1,2-benzenedicarboxylic acid, mono(2-ethylhexyl) ester. On the basis of morphological characters and rDNA sequencing of ITS region the endophyte was identified as Aspergillus flavipes which showed promising plant growth promotory properties

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