The Proteome of the Differentiating Mesencephalic Progenitor Cell Line CSM14.1In Vitro | Zendy

B Weiss | Zendy; Stefan Haas | Zendy; Grit Lessner | Zendy; Stefan Mikkat | Zendy; Michael Kreutzer | Zendy; Michael O. Glocker | Zendy; Andreas Wree | Zendy; Oliver Schmitt | Zendy

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The Proteome of the Differentiating Mesencephalic Progenitor Cell Line CSM14.1In Vitro

Author(s) -

B Weiss,

Stefan Haas,

Grit Lessner,

Stefan Mikkat,

Michael Kreutzer,

Michael O. Glocker,

Andreas Wree,

Oliver Schmitt

Publication year - 2014

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2014/351821

Subject(s) - progenitor cell , biology , microbiology and biotechnology , embryonic stem cell , cellular differentiation , proteome , cell culture , cell type , stem cell , nestin , tyrosine hydroxylase , transplantation , proteomics , cell , neural stem cell , immunology , bioinformatics , genetics , medicine , immunohistochemistry , gene

The treatment of Parkinson's disease by transplantation of dopaminergic (DA) neurons from human embryonic mesencephalic tissue is a promising approach. However, the origin of these cells causes major problems: availability and standardization of the graft. Therefore, the generation of unlimited numbers of DA neurons from various types of stem or progenitor cells has been brought into focus. A source for DA neurons might be conditionally immortalized progenitor cells. The temperature-sensitive immortalized cell line CSM14.1 derived from the mesencephalon of an embryonic rat has been used successfully for transplantation experiments. This cell line was analyzed by unbiased stereology of cell type specific marker proteins and 2D-gel electrophoresis followed by mass spectrometry to characterize the differentially expressed proteome. Undifferentiated CSM14.1 cells only expressed the stem cell marker nestin, whereas differentiated cells expressed GFAP or NeuN and tyrosine hydroxylase. An increase of the latter cells during differentiation could be shown. By using proteomics an explanation on the protein level was found for the observed changes in cell morphology during differentiation, when CSM14.1 cells possessed the morphology of multipolar neurons. The results obtained in this study confirm the suitability of CSM14.1 cells as an in vitro model for the study of neuronal and dopaminergic differentiation in rats.

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