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Development of a Novel Cysteine Sulfinic Acid Decarboxylase Knockout Mouse: Dietary Taurine Reduces Neonatal Mortality
Author(s) -
Eunkyue Park,
SeungYong Park,
Carl Dobkin,
Georgia SchullerLevis
Publication year - 2014
Publication title -
journal of amino acids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.188
H-Index - 5
eISSN - 2090-0112
pISSN - 2090-0104
DOI - 10.1155/2014/346809
Subject(s) - taurine , sulfinic acid , medicine , cysteine , knockout mouse , biochemistry , pharmacology , enzyme , amino acid , chemistry , receptor , organic chemistry
We engineered a CSAD KO mouse to investigate the physiological roles of taurine. The disruption of the CSAD gene was verified by Southern, Northern, and Western blotting. HPLC indicated an 83% decrease of taurine concentration in the plasma of CSAD −/− . Although CSAD −/− generation (G)1 and G2 survived, offspring from G2 CSAD −/− had low brain and liver taurine concentrations and most died within 24 hrs of birth. Taurine concentrations in G3 CSAD −/− born from G2 CSAD −/− treated with taurine in the drinking water were restored and survival rates of G3 CSAD −/− increased from 15% to 92%. The mRNA expression of CDO, ADO, and TauT was not different in CSAD −/− compared to WT and CSAD mRNA was not expressed in CSAD −/− . Expression of Gpx 1 and 3 was increased significantly in CSAD −/− and restored to normal levels with taurine supplementation. Lactoferrin and the prolactin receptor were significantly decreased in CSAD −/− . The prolactin receptor was restored with taurine supplementation. These data indicated that CSAD KO is a good model for studying the effects of taurine deficiency and its treatment with taurine supplementation.

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