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Background Information . NF-  κ  B signaling pathway plays a complicated role in the biological functions of mesenchymal stem cells. However, the effects of NF-  κ  B pathway on the odonto/osteogenic differentiation of stem cells from apical papilla (SCAPs) remain unclear. The present study was designed to evaluate the effects of canonical NF-  κ  B pathway on the osteo/odontogenic capacity of SCAPs  in vitro .  Results . Western blot results demonstrated that NF-  κ  B pathway in SCAPs was successfully activated by TNF-  α   or blocked by BMS-345541. NF-  κ  B pathway-activated SCAPs presented a higher proliferation activity compared with control groups, as indicated by dimethyl-thiazol-diphenyl tetrazolium bromide assay (MTT) and flow cytometry assay (FCM). Wound scratch assay revealed that NF-  κ  B pathway-activated SCAPs presented an improved migration capacity, enhanced alkaline phosphatase (ALP) activity, and upregulated mineralization capacity of SCAPs, as compared with control groups. Meanwhile, the odonto/osteogenic markers ( ALP /ALP,  RUNX2 /RUNX2,  OSX /OSX,  OCN /OCN,  OPN /OPN,  BSP /BSP,  DSPP /DSP, and  DMP-1 /DMP-1) in NF-  κ  B pathway-activated SCAPs were also significantly upregulated as compared with control groups at both protein and mRNA levels. However, NF-  κ  B pathway-inhibited SCAPs exhibited a lower proliferation/migration capacity, and decreased odonto/osteogenic ability in comparison with control groups.  Conclusion . Our findings suggest that classical NF-  κ  B pathway plays a paramount role in the proliferation and committed differentiation of SCAPs.

Effects of Canonical NF-κB Signaling Pathway on the Proliferation and Odonto/Osteogenic Differentiation of Human Stem Cells from Apical Papilla