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Objective.  To evaluate bacterial growth inhibition, mechanical properties, and compound release rate and stability of copolymers incorporated with anthocyanin (ACY;  Vaccinium macrocarpon ).  Methods.  Resin samples were prepared (Bis-GMA/TEGDMA at 70/30 mol%) and incorporated with 2 w/w% of either ACY or chlorhexidine (CHX), except for the control group. Samples were individually immersed in a bacterial culture ( Streptococcus mutans ) for 24 h. Cell viability ( n  = 3) was assessed by counting the number of colony forming units on replica agar plates. Flexural strength (FS) and elastic modulus ( E ) were tested on a universal testing machine ( n  = 8). Compound release and chemical stability were evaluated by UV spectrophotometry and  1 H NMR ( n  = 3). Data were analyzed by one-way ANOVA and Tukey's test (  α   = 0.05).  Results.  Both compounds inhibited S. mutans growth, with CHX being most effective ( P  < 0.05). Control resin had the lowest FS and E values, followed by ACY and CHX, with statistical difference between control and CHX groups for both mechanical properties ( P  < 0.05). The 24 h compound release rates were ACY: 1.33  μ g/mL and CHX: 1.92  μ g/mL.  1 H NMR spectra suggests that both compounds remained stable after being released in water.  Conclusion.  The present findings indicate that anthocyanins might be used as a natural antibacterial agent in resin based materials.

international journal of dentistry

Anthocyanin Incorporated Dental Copolymer: Bacterial Growth Inhibition, Mechanical Properties, and Compound Release Rates and Stability by<sup>1</sup>H NMR

Anthocyanin Incorporated Dental Copolymer: Bacterial Growth Inhibition, Mechanical Properties, and Compound Release Rates and Stability by1H NMR

Anthocyanin Incorporated Dental Copolymer: Bacterial Growth Inhibition, Mechanical Properties, and Compound Release Rates and Stability by¹H NMR