Establishment of a Tongue Squamous Cell Carcinoma Cell Line from Indian Gutka Chewer | Zendy

Tejas Patil | Zendy; Pradnya Kowtal | Zendy; Abhijeet Nikam | Zendy; Madan Barkume | Zendy; Asawari Patil | Zendy; Shubhada Kane | Zendy; Aarti Juvekar | Zendy; Manoj B. Mahimkar | Zendy; J. J. Kayal | Zendy

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Establishment of a Tongue Squamous Cell Carcinoma Cell Line from Indian Gutka Chewer

Author(s) -

Tejas Patil,

Pradnya Kowtal,

Abhijeet Nikam,

Madan Barkume,

Asawari Patil,

Shubhada Kane,

Aarti Juvekar,

Manoj B. Mahimkar,

J. J. Kayal

Publication year - 2014

Publication title -

journal of oral oncology

Language(s) - English

Resource type - Journals

eISSN - 2356-7554

pISSN - 2314-6591

DOI - 10.1155/2014/286013

Subject(s) - cell culture , biology , cell , retinoic acid , karyotype , cisplatin , missense mutation , cell growth , comparative genomic hybridization , cancer research , microbiology and biotechnology , ultrastructure , phenotype , genetics , chromosome , gene , anatomy , chemotherapy

CD cell line has been established from a poorly differentiated squamous cell carcinoma of tongue. This is a first ever cell line established from an Indian gutka chewer. Cell line was characterized for morphology, ultrastructure, doubling time, expression of epithelial markers, DNA content, karyotyping, STR markers, p53 mutations, HPV status, and tumorigenicity in SCID mice with all-trans-retinoic acid and cisplatin. The epithelial phenotype of the cell line was confirmed with surface markers and ultrastructure. The cell line is hyperploid with chromosomal alterations like gain of chromosomes 8q and 11q. CD cell line shows a unique pattern on STR genotyping and carries a missense mutation R273C in TP53. It does not show genomic integration of HPV. The cells are nontumorigenic to SCID mice and show growth inhibition upon treatment with cisplatin, and all-trans-retinoic acid. This cell line may be useful as an in vitro tool to understand the molecular changes associated with oral cancers.

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