Determination of (4E,6E,12E)-Tetradecatriene-8,10-diyne-1,3-diyl Diacetate in Rat Plasma and Tissues by HPLC-UV Method and Their Application to a Pharmacokinetic and Tissue Distribution Study

In China Atractylodis Rhizoma is widely used for the treatment of rheumatic diseases and digestive disorders. Stir-frying with wheat bran is the most common processing method. In order to clarify the influence of processing on pharmacological properties of Atractylodis Rhizoma , an investigation was carried out to compare the pharmacokinetics and tissue distribution of typical constituent after oral administration of raw Atractylodis Rhizoma and processed ones. A simple, rapid, and sensitive high performance liquid chromatography with UV detection was developed and validated for the determination of (4 E ,6 E ,12 E )-tetradecatriene-8,10-diyne-1,3-diyl diacetate in rat plasma. A chromatography was carried out on Diamonsil C 18 (250 × 4.6 mm; 5  μ m) analytical column, using a mobile phase which consisted of acetonitrile and 0.1% phosphoric acid water (60 : 40, v/v) at a flow rate of 1.0 mL · min −1 . The wavelength was set at 336 nm. The LLOQ of (4 E ,6 E ,12 E )-tetradecatriene-8,10-diyne-1,3-diyl diacetate was 0.00143  μ g · mL −1 . Both accuracy and precision were satisfactory. The pharmacokinetic results showed that the T max⁡ was 1 hour in advance and the C max⁡ was increased after processing. Tissue distribution showed that the highest level was in spleen. And the concentrations in the spleen were increased after stir-frying with bran.