Promoter Region Hypermethylation and mRNA Expression ofMGMTandp16Genes in Tissue and Blood Samples of Human Premalignant Oral Lesions and Oral Squamous Cell Carcinoma | Zendy

Vikram Bhatia | Zendy; Madhu Mati Goel | Zendy; Annu Makker | Zendy; Shikha Tewari | Zendy; Alka Yadu | Zendy; Priyanka Shilpi | Zendy; Sandeep Kumar | Zendy; Saurabh Agarwal | Zendy; Sudhir K. Goel | Zendy

Open Access

Promoter Region Hypermethylation and mRNA Expression ofMGMTandp16Genes in Tissue and Blood Samples of Human Premalignant Oral Lesions and Oral Squamous Cell Carcinoma

Author(s) -

Vikram Bhatia,

Madhu Mati Goel,

Annu Makker,

Shikha Tewari,

Alka Yadu,

Priyanka Shilpi,

Sandeep Kumar,

Saurabh Agarwal,

Sudhir K. Goel

Publication year - 2014

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2014/248419

Subject(s) - dna methylation , methylation , methyltransferase , cancer research , cancer , malignancy , gene silencing , biology , gene , pathology , carcinoma , gene expression , medicine , genetics

Promoter methylation and relative gene expression of O 6 -methyguanine-DNA-methyltransferase ( MGMT ) and p16 genes were examined in tissue and blood samples of patients with premalignant oral lesions (PMOLs) and oral squamous cell carcinoma (OSCC). Methylation-specific PCR and reverse transcriptase PCR were performed in 146 tissue and blood samples from controls and patients with PMOLs and OSCC. In PMOL group, significant promoter methylation of MGMT and p16 genes was observed in 59% ( P = 0.0010) and 57% ( P = 0.0016) of tissue samples, respectively, and 39% ( P = 0.0135) and 33% ( P = 0.0074) of blood samples, respectively. Promoter methylation of both genes was more frequent in patients with OSCC, that is, 76% ( P = 0.0001) and 82% ( P = 0.0001) in tissue and 57% ( P = 0.0002) and 70% ( P = 0.0001) in blood, respectively. Significant downregulation of MGMT and p16 mRNA expression was observed in both tissue and blood samples from patients with PMOLs and OSCC. Hypermethylation-induced transcriptional silencing of MGMT and p16 genes in both precancer and cancer suggests important role of these changes in progression of premalignant state to malignancy. Results support use of blood as potential surrogate to tissue samples for screening or diagnosing PMOLs and early OSCC.

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