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Objective.  The aim of this study was to elucidate the mechanism(s) responsible for the vasorelaxant effect of  Nigella sativa  ( N. sativa ).  Methods.  The activity of different concentrations of  N. sativa  extract was evaluated on contractile responses of isolated aorta to KCl and phenylephrine (PE).  Results.  The extract (2–14 mg/mL) induced a concentration dependent relaxation both in endothelium-intact and endothelium-denuded aortic rings precontracted by PE (10 −6  M) and KCl (6 × 10 −2  M). Extract reduced PE- and KCl-induced contractions in presence of cumulative concentrations of calcium (10 −5 –10 −2  M) significantly. L-NAME and indomethacin had no effect on vasorelaxation effect of extract in PE-induced contraction. Diltiazem and heparin reduced significantly this vasorelaxation at a concentration of 14 mg/mL of extract; however,  N. sativa -induced relaxation was not affected by ruthenium red. Tetraethylammonium chloride reduced the extract-induced relaxation in concentrations of 2–6 mg/mL of extract significantly but glibenclamide reduced this relaxative effect in all concentrations of extract.  Conclusions.  The inhibitory effect of  N. sativa  seed extract on the contraction induced by PE and KCl was endothelium-independent. This relaxation was mediated mainly through the inhibition of Ca 2+  and K ATP  channels and also intracellular calcium release.

biomed research international

Endothelium-Independent Vasorelaxant Effects of Hydroalcoholic Extract fromNigella sativaSeed in Rat Aorta: The Roles of Ca2+and K+Channels