Evaluation of Production Parameters for Maximum Lipase Production by P. stutzeri MTCC 5618 and Scale-Up in Bioreactor | Zendy

Vishal Thakur | Zendy; Rupinder Tewari | Zendy; Rohit Sharma | Zendy

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Evaluation of Production Parameters for Maximum Lipase Production by P. stutzeri MTCC 5618 and Scale-Up in Bioreactor

Author(s) -

Vishal Thakur,

Rupinder Tewari,

Rohit Sharma

Publication year - 2014

Publication title -

chinese journal of biology

Language(s) - English

Resource type - Journals

ISSN - 2314-7474

DOI - 10.1155/2014/208462

Subject(s) - lipase , pseudomonas stutzeri , bioreactor , fermentation , chemistry , food science , aeration , pulmonary surfactant , hydrolysis , chromatography , biochemistry , enzyme , biology , bacteria , organic chemistry , genetics

Intracellular lipase producer screened from the library available in the laboratory, identified through 16S rRNA as Pseudomonas stutzeri, was studied for maximum enzyme production in shake flask. The work was intended to evaluate the effect of different physicochemical factors like carbon, nitrogen, metal ions, surfactant, inoculum, pH, temperature, agitation, and aeration on lipase production. Optimized media showed 1.62-fold increase in lipase production when compared to basal media. Scale-up of lipase in in situ bioreactor showed reduction in fermentation time in both basal and optimized media, giving 41 and 99 U/mg of lipase activity after 48 h of fermentation

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