Enhanced Osteogenicity of Bioactive Composites with Biomimetic Treatment | Zendy

Ville V. Meretoja | Zendy; Teemu Tirri | Zendy; Minna Malin | Zendy; Jukka Seppälä | Zendy; Timo Närhi | Zendy

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Enhanced Osteogenicity of Bioactive Composites with Biomimetic Treatment

Author(s) -

Ville V. Meretoja,

Teemu Tirri,

Minna Malin,

Jukka Seppälä,

Timo Närhi

Publication year - 2014

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2014/207676

Subject(s) - simulated body fluid , alkaline phosphatase , osteocalcin , apatite , bone sialoprotein , bioactive glass , calcium , materials science , osteoblast , mineralization (soil science) , membrane , composite number , polymer , chemistry , composite material , biomedical engineering , mineralogy , biochemistry , organic chemistry , medicine , in vitro , nitrogen , metallurgy , enzyme

Purpose . This study aimed to explore if initiation of biomimetic apatite nucleation can be used to enhance osteoblast response to biodegradable tissue regeneration composite membranes. Materials and Methods . Bioactive thermoplastic composites consisting of poly( ε -caprolactone/DL-lactide) and bioactive glass (BAG) were prepared at different stages of biomimetic calcium phosphate deposition by immersion in simulated body fluid (SBF). The modulation of the BAG dissolution and the osteogenic response of rat mesenchymal stem cells (MSCs) were analyzed. Results . SBF treatment resulted in a gradual calcium phosphate deposition on the composites and decreased BAG reactivity in the subsequent cell cultures. Untreated composites and composites covered by thick calcium phosphate layer (14 days in SBF) expedited MSC mineralization in comparison to neat polymers without BAG, whereas other osteogenic markers—alkaline phosphatase activity, bone sialoprotein, and osteocalcin expression—were initially decreased. In contrast, surfaces with only small calcium phosphate aggregates (five days in SBF) had similar early response than neat polymers but still demonstrated enhanced mineralization. Conclusion . A short biomimetic treatment enhances osteoblast response to bioactive composite membranes.

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