Optimization of Fluorometric Assay for Ozone in Solution | Zendy

Hiromi Nagano | Zendy; Gotaro Shiota | Zendy; Hidetoshi Arakawa | Zendy

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Optimization of Fluorometric Assay for Ozone in Solution

Author(s) -

Hiromi Nagano,

Gotaro Shiota,

Hidetoshi Arakawa

Publication year - 2014

Publication title -

journal of analytical methods in chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.407

H-Index - 25

eISSN - 2090-8865

pISSN - 2090-8873

DOI - 10.1155/2014/190937

Subject(s) - ozone , fluorescein , fluorescence , chemistry , sterilization (economics) , derivative (finance) , environmental chemistry , environmental science , chromatography , organic chemistry , optics , physics , monetary economics , economics , foreign exchange market , foreign exchange , financial economics

Ozone is widely used for sterilization, deodorization, and cleaning. However, the effects of ozone on human are not well understood, because there is currently no reliable assay method for ozone. Therefore the accurate measurement of ozone is important for its safe use. Here, we report optimized conditions for use with fluorescein derivative 1 (probe 1 ). Under optimum conditions, probe 1 reacted with ozone quantitatively and specifically to generate a fluorescent substance with an excitation maximum at 493 nm and an emission maximum at 523 nm. We conclude that this assay will be useful for quantifying ozone in solution.

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