Combined Cytogenotoxic Effects of Bee Venom and Bleomycin on Rat Lymphocytes: AnIn VitroStudy | Zendy

Yasmina M. AbdElhakim | Zendy; Samah R. Khalil | Zendy; Ashraf Awad | Zendy; Laila AlAyadhi | Zendy

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Combined Cytogenotoxic Effects of Bee Venom and Bleomycin on Rat Lymphocytes: AnIn VitroStudy

Author(s) -

Yasmina M. AbdElhakim,

Samah R. Khalil,

Ashraf Awad,

Laila AlAyadhi

Publication year - 2014

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 126

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/2014/173903

Subject(s) - cytotoxicity , ficoll , bleomycin , dna fragmentation , microbiology and biotechnology , apoptosis , mtt assay , dna damage , biology , in vitro , cytotoxic t cell , genotoxicity , pharmacology , chemistry , dna , programmed cell death , toxicity , biochemistry , peripheral blood mononuclear cell , chemotherapy , genetics , organic chemistry

This study was carried out to determine the cytotoxic and genotoxic effects of bee venom (BV) and/or the chemotherapeutic agent bleomycin (BLM) on healthy isolated rat lymphocytes utilizing morphometric and molecular techniques. Using the Ficoll-Histopaque density gradient centrifugation technique, lymphocytes were isolated, divided into groups, and subjected to BV and/or BLM at incubation medium concentrations of 10 or 20 μ g/mL respectively for 24 and 72 hrs. An MTT assay and fluorescent microscopy examinations were used to assess the cytotoxic effects. To determine the predominant type of BV and/or BLM-induced cell death, LDH release assay was employed beside quantitative expression analyses of the apoptosis-related genes (Caspase-3 and Bcl-2). The genotoxic effects of the tested compounds were evaluated via DNA fragmentation assay. The results of these assays demonstrated that BV potentiates BLM-induced cytotoxicity through increased LDH release and diminished cell viability. Nevertheless, BV significantly inhibited the BLM-induced DNA damage. The results verify that BV significantly attenuates the genotoxic effects of BLM on noncancerous isolated rat lymphocytes but does not diminish BLM cytotoxicity.

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