English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

T. reesei  is an efficient cellulase producer and biomass degrader. To improve xylitol production in  Trichoderma reesei  strains by genetic engineering, two approaches were used in this study. First, the presumptive D-xylulokinase gene in   T. reesei  ( xyiH ), which has high homology to known fungi D-xylulokinase genes, was silenced by transformation of   T. reesei  QM9414 strain with an antisense construct to create strain S6-2-2. The expression of the   xyiH  gene in the transformed strain S6-2-2 decreased at the mRNA level, and D-xylulokinase activity decreased after 48 h of incubation. This led to an increase in xylitol production from undetectable levels in wild-type   T. reesei  QM9414 to 8.6 mM in S6-2-2. The   T. reesei  Δxdh is a xylose dehydrogenase knockout strain with increased xylitol production compared to the wild-type   T. reesei  QM9414 (22.8 mM versus undetectable). The copy number of the xylose reductase gene ( xyl 1) in   T. reesei   Δxdh strain was increased by genetic engineering to create a new strain Δ9-5-1. The Δ9-5-1 strain showed a higher   xyl 1 expression and a higher yield of xylose reductase, and xylitol production was increased from 22.8 mM to 24.8 mM. Two novel strains S6-2-2 and Δ9-5-1 are capable of producing higher yields of xylitol.   T. reesei  has great potential in the industrial production of xylitol.

biomed research international

Overexpression of D-Xylose Reductase (xyl1) Gene and Antisense Inhibition of D-Xylulokinase (xyiH) Gene Increase Xylitol Production inTrichoderma reesei