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Analysis of a Benzamide/Cholesterol Mixture by Using TLC/FTIR Technique
Author(s) -
Xiaokun Fan,
Ran Guo,
Jiajia Shi,
Haijun Wu,
Anqi He,
Yongju Wei,
Cuige Liu,
Shifu Weng,
Zhanlan Yang,
Yizhuang Xu,
Isao Noda,
Jinguang Wu
Publication year - 2013
Publication title -
journal of spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.323
H-Index - 21
eISSN - 2314-4920
pISSN - 2314-4939
DOI - 10.1155/2013/976360
Subject(s) - benzamide , fourier transform infrared spectroscopy , chemistry , chromatography , analytical chemistry (journal) , silica gel , stereochemistry , optics , physics
We applied TLC/FTIR coupled with a mapping technique to analyze a cholesterol/benzamide mixture. Narrow-band TLC plates by using AgI as a stationary phase were used to separate benzamide and cholesterol. The distribution of cholesterol and benzamide spots was manifested by 3D chromatogram. Benzamide and cholesterol can be successfully separated by the narrow-band TLC plate. Moreover, characteristic bands of benzamide and cholesterol can be identified from their FTIR spectra. In addition, the FTIR spectra of the separated benzamide and cholesterol spots on the narrow band TLC plate are roughly the same as the corresponding reference IR spectra. © 2013 Xiaokun Fan et al.

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