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Background .  Lactobacillus  shows beneficial anti-inflammatory effects on  Salmonella  infection. The maintenance of the tight junction (TJ) integrity plays an importance role in avoiding bacterial invasion. Whether  Lactobacillus  could be used to regulate the TJ protein expression and distribution in inflamed intestinal epithelial cells was determined.  Methods . Using the transwell coculture model,  Salmonella  lipopolysaccharide (LPS) was apically added to polarized Caco-2 cells cocultured with peripheral blood mononuclear cells in the basolateral compartment. LPS-stimulated Caco-2 cells were incubated with various  Lactobacillus  strains. TJ integrity was determined by measuring transepithelial electrical resistance across Caco-2 monolayer. Expression and localization of TJ proteins (zonula-occludens- (ZO-) 1) were determined by Western blot and immunofluorescence microscopy.  Results . Various strains of  Lactobacillus  were responsible for the different modulations of cell layer integrity. LPS was specifically able to disrupt epithelial barrier and change the location of ZO-1. Our data demonstrate that  Lactobacillus  could attenuate the barrier disruption of intestinal epithelial cells caused by  Salmonella  LPS administration. We showed that  Lactobacillus  strains are associated with the maintenance of the tight junction integrity and appearance.  Conclusion . In this study we provide insight that live probiotics could improve epithelial barrier properties and this may explain the potential mechanism behind their beneficial effect  in vivo .

In VitroPrevention ofSalmonellaLipopolysaccharide-Induced Damages in Epithelial Barrier Function by VariousLactobacillusStrains