English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Calcium signals in hepatocytes control cell growth, proliferation, and death. Members of the transient receptor potential (TRP) cation channel superfamily are candidate calcium influx channels. NF  κ  B activation strictly depends on calcium influx and often induces antiapoptotic genes favouring cell survival. Previously, we reported that  S -[6]-gingerol is an efficacious agonist of the transient receptor potential cation channel subfamily V member 1 (TRPV1) in neurones. In this study, we tested the effect of  S -[6]-gingerol on HuH-7 cells using the Fluo-4 calcium assay, RT-qPCR, transient cell transfection, and luciferase measurements. We found that  S -[6]-gingerol induced a transient rise in [Ca 2+ ]  i   in HuH-7 cells. The increase in [Ca 2+ ]  i   induced by  S -[6]-gingerol was abolished by preincubation with EGTA and was also inhibited by the TRPV1 channel antagonist capsazepine. Expression of TRPV1 in HuH-7 cells was confirmed by mRNA analysis as well as a test for increase of [Ca 2+ ]  i   by TRPV1 agonist capsaicin and its inhibition by capsazepine. We found that  S -[6]-gingerol induced rapid NF  κ  B activation through TRPV1 in HuH-7 cells. Furthermore,  S -[6]-gingerol-induced NF  κ  B activation was dependent on the calcium gradient and TRPV1. The rapid NF  κ  B activation by  S -[6]-gingerol was associated with an increase in mRNA levels of NF  κ  B-target genes: cIAP-2, XIAP, and Bcl-2 that encode antiapoptotic proteins.

Identification of a Calcium Signalling Pathway ofS-[6]-Gingerol in HuH-7 Cells