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A sensitive and specific gas chromatographic-mass spectrometry with selected ion monitoring (GC-MS/SIM) method has been developed for simultaneous identification and quantification of   α  -asarone,  β -asarone, and methyl eugenol of  Acorus tatarinowii  Schott in rat plasma. Chromatographic separation was performed on a Restek Rxi-5MS capillary column (30 m × 0.32 mm × 0.25  μ m), using 1-naphthol as internal standard (IS). MS detection of these compounds and IS was performed at  m/z  178, 208, 208, and 144. Intra- and interday precisions of all compounds of interest were less than 10%. The recoveries are situated in the range of 92.4–105.2%. Pharmacokinetics of methyl eugenol confirmed to be one-compartment open model,   α  -asarone and   β  -asarone was two-compartment open model, respectively. The method will probably be an alternative to simultaneous determination and pharmacokinetic study of volatile ingredients in  Acorus tatarinowii  Schott.

journal of analytical methods in chemistry

Simultaneous Determination of Volatile Constituents from<i>Acorus tatarinowii</i>Schott in Rat Plasma by Gas Chromatography-Mass Spectrometry with Selective Ion Monitoring and Application in Pharmacokinetic Study

Simultaneous Determination of Volatile Constituents fromAcorus tatarinowiiSchott in Rat Plasma by Gas Chromatography-Mass Spectrometry with Selective Ion Monitoring and Application in Pharmacokinetic Study