Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture | Zendy

Masumi Akita | Zendy; Kayoko Tanaka | Zendy; Sachiko Matsumoto | Zendy; Kumiko Komatsu | Zendy; Keiko Fujita | Zendy

Open Access

Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture

Author(s) -

Masumi Akita,

Kayoko Tanaka,

Sachiko Matsumoto,

Kumiko Komatsu,

Keiko Fujita

Publication year - 2013

Publication title -

stem cells international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.205

H-Index - 64

eISSN - 1687-9678

pISSN - 1687-966X

DOI - 10.1155/2013/927403

Subject(s) - angiogenesis , progenitor cell , immunogold labelling , explant culture , haematopoiesis , endothelial stem cell , microbiology and biotechnology , stem cell , chemistry , in vitro , population , biology , immunology , medicine , cancer research , biochemistry , antibody , environmental health

We detected the hematopoietic stem and progenitor cell marker CD133 using immunogold labeling during angiogenesis in a three-dimensional collagen gel culture. CD133-positive cells were present in capillary tubes newly formed from aortic explants in vitro. The CD133-positive cell population had the capacity to form capillary tubes. Lovastatin strongly inhibited cell migration from aortic explants and caused the degradation of the capillary tubes. The present study provides insight into the function of CD133 during angiogenesis as well as an explanation for the antiangiogenic effect of statins.

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