A New Multichannel Spectral Imaging Laser Scanning Confocal Microscope
Author(s) -
Yunhai Zhang,
Bian Hu,
Yakang Dai,
Haomin Yang,
Wei Huang,
Xiaojun Xue,
Fazhi Li,
Xin Zhang,
Chenyu Jiang,
Fei Gao,
Jian Chang
Publication year - 2013
Publication title -
computational and mathematical methods in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.462
H-Index - 48
eISSN - 1748-6718
pISSN - 1748-670X
DOI - 10.1155/2013/890203
Subject(s) - confocal , microscope , spectral imaging , laser scanning , confocal laser scanning microscope , confocal microscopy , fluorescence , confocal laser scanning microscopy , optics , laser , fluorescence lifetime imaging microscopy , optical sectioning , microscopy , materials science , biomedical engineering , physics , medicine
We have developed a new multichannel spectral imaging laser scanning confocal microscope for effective detection of multiple fluorescent labeling in the research of biological tissues. In this paper, the design and key technologies of the system are introduced. Representative results on confocal imaging, 3-dimensional sectioning imaging, and spectral imaging are demonstrated. The results indicated that the system is applicable to multiple fluorescent labeling in biological experiments.
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