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Some studies have shown that the NetB toxin may be an important virulence factor of  Clostridium perfringens  associated necrotic enteritis in poultry. Additionally, research has shown that strains of  C. perfringens  positive for both the  netB  gene and a second toxin-encoding gene,  tpeL , appear to be more virulent than strains with only  netB . In the past, detection of these genes has been performed relatively inefficiently using two single locus PCRs. This report describes a novel multiplex PCR developed to detect  netB  and  tpeL  simultaneously in  C. perfringens  strains isolated from cases of necrotic enteritis in broilers, providing a more efficient diagnostic tool in the screening of strains for these genes.

Use of a Multiplex PCR for the Detection of Toxin-Encoding Genes netB and tpeL in Strains of Clostridium perfringens