Comparison of Uric Acid Quantity with Different Food in Human Urine by Flow Injection Chemiluminescence Analysis | Zendy

Jiajia Wang | Zendy; Xijuan Tan | Zendy; Zhenghua Song | Zendy

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Comparison of Uric Acid Quantity with Different Food in Human Urine by Flow Injection Chemiluminescence Analysis

Author(s) -

Jiajia Wang,

Xijuan Tan,

Zhenghua Song

Publication year - 2013

Publication title -

journal of analytical methods in chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.407

H-Index - 25

eISSN - 2090-8865

pISSN - 2090-8873

DOI - 10.1155/2013/854041

Subject(s) - chemiluminescence , uric acid , urine , flow injection analysis , chemistry , food science , chromatography , biochemistry , detection limit

Based on the inhibitory effect of uric acid (UA) on luminol-Co 2+ chemiluminescence (CL) system, a sensitive method for the determination of UA at nanomolar level by flow injection (FI) CL was proposed. The proposed method was successfully applied to real-time monitoring of UA excretion in human 24 h urine with different food intake, showing that meats, vegetables, and porridge intake caused differential UA excretions of 879, 798, and 742 mg, respectively. It was also found that UA concentrations in urine under the three kinds of food intake simultaneously reached maximum at 2 h after meals with the values of 417, 318, and 288 μ g mL −1 , respectively. The UA concentration in human serum was also determined by this approach, and the possible mechanism of luminol-Co 2+ -UA CL reaction was discussed in detail.

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