Analysis of the Kanamycin in Raw Milk Using the Suspension Array | Zendy

Yanfei Wang | Zendy; Mingqiang Zou | Zendy; Yi Ping Han | Zendy; Fan Zhang | Zendy; Jinfeng Li | Zendy; Xiaoyan Zhu | Zendy

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Analysis of the Kanamycin in Raw Milk Using the Suspension Array

Author(s) -

Yanfei Wang,

Mingqiang Zou,

Yi Ping Han,

Fan Zhang,

Jinfeng Li,

Xiaoyan Zhu

Publication year - 2012

Publication title -

journal of chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.436

H-Index - 50

eISSN - 2090-9063

pISSN - 2090-9071

DOI - 10.1155/2013/784543

Subject(s) - kanamycin , chemistry , raw milk , chromatography , aminoglycoside , detection limit , monoclonal antibody , immunoassay , suspension (topology) , antibiotics , antibody , food science , biochemistry , immunology , mathematics , homotopy , pure mathematics , biology

With the monoclonal antibody against kanamycin being prepared successfully, a bead-based indirect competitive fluorescent immunoassay was developed to detect kanamycin in milk. The fact that there was no significant cross-reaction with other aminoglycoside antibiotics implied that the monoclonal antibody was highly specific for kanamycin. The limit of detection (LOD) and the 50 inhibition concentration (IC in raw milk were 3.2 ng/mL and 52.5 ng/mL, respectively. Using the method developed in this study, the kanamycin concentrations were monitored in raw milk after the intramuscular administration of kanamycin in sick cows. Compared to the conventional enzyme-linked immunosorbent assay (ELISA), the method using the suspension array system was more sensitive. The results obtained in the present study showed a good correlation with that of the ELISA. © 2013 Yanfei Wang et al.

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