Evaluation of a Propolis Water Extract Using a Reliable RP-HPLC Methodology andIn VitroandIn VivoEfficacy and Safety Characterisation | Zendy

Bruno Alves Rocha | Zendy; Paula Carolina Pires Bueno | Zendy; Mirela Mara de Oliveira Lima Leite Vaz | Zendy; Andresa Piacezzi Nascimento | Zendy; Nathália Ursoli Ferreira | Zendy; Gabriela Moreno | Zendy; Marina Rezende Rodrigues | Zendy; Ana Rita de Mello Costa-Machado | Zendy; Edna Aparecida Barizon | Zendy; Jacqueline Costa Lima Campos | Zendy; Pollyanna Francielli de Oliveira | Zendy; Nathália Acésio | Zendy; Sabrina de Paula Lima Martins | Zendy; Denise Crispim Tavares | Zendy; Andresa Aparecida Berretta | Zendy

Open Access

Evaluation of a Propolis Water Extract Using a Reliable RP-HPLC Methodology andIn VitroandIn VivoEfficacy and Safety Characterisation

Author(s) -

Bruno Alves Rocha,

Paula Carolina Pires Bueno,

Mirela Mara de Oliveira Lima Leite Vaz,

Andresa Piacezzi Nascimento,

Nathália Ursoli Ferreira,

Gabriela Moreno,

Marina Rezende Rodrigues,

Ana Rita de Mello Costa-Machado,

Edna Aparecida Barizon,

Jacqueline Costa Lima Campos,

Pollyanna Francielli de Oliveira,

Nathália Acésio,

Sabrina de Paula Lima Martins,

Denise Crispim Tavares,

Andresa Aparecida Berretta

Publication year - 2013

Publication title -

evidence-based complementary and alternative medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.552

H-Index - 90

eISSN - 1741-4288

pISSN - 1741-427X

DOI - 10.1155/2013/670451

Subject(s) - propolis , antimicrobial , in vivo , micronucleus test , antioxidant , micronucleus , chemistry , in vitro , traditional medicine , in vitro toxicology , antifungal , pharmacology , toxicity , high performance liquid chromatography , chromatography , biology , food science , microbiology and biotechnology , medicine , biochemistry , organic chemistry

Since the beginning of propolis research, several groups have studied its antibacterial, antifungal, and antiviral properties. However, most of these studies have only employed propolis ethanolic extract (PEE) leading to little knowledge about the biological activities of propolis water extract (PWE). Based on this, in a previous study, we demonstrated the anti-inflammatory and immunomodulatory activities of PWE. In order to better understand the equilibrium between effectiveness and toxicity, which is essential for a new medicine, the characteristics of PWE were analyzed. We developed and validated an RP-HPLC method to chemically characterize PWE and PEE and evaluated the in vitro antioxidant/antimicrobial activity for both extracts and the safety of PWE via determining genotoxic potential using in vitro and in vivo mammalian micronucleus assays. We have concluded that the proposed analytical methodology was reliable, and both extracts showed similar chemical composition. The extracts presented antioxidant and antimicrobial effects, while PWE demonstrated higher antioxidant activity and more efficacious for the most of the microorganisms tested than PEE. Finally, PWE was shown to be safe using micronucleus assays.

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