Variable-Temperature Size Exclusion Chromatography for the Study of the Structural Changes in G-Quadruplex | Zendy

Sanae Benabou | Zendy; Ramón Eritja | Zendy; Raimundo Gargallo | Zendy

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Variable-Temperature Size Exclusion Chromatography for the Study of the Structural Changes in G-Quadruplex

Author(s) -

Sanae Benabou,

Ramón Eritja,

Raimundo Gargallo

Publication year - 2013

Publication title -

isrn biochemistry

Language(s) - English

Resource type - Journals

ISSN - 2090-7729

DOI - 10.1155/2013/631875

Subject(s) - antiparallel (mathematics) , circular dichroism , monomer , size exclusion chromatography , chemistry , conformational change , g quadruplex , sequence (biology) , crystallography , guanine , stereochemistry , dna , polymer , biochemistry , nucleotide , gene , organic chemistry , physics , enzyme , quantum mechanics , magnetic field

The conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature size exclusion chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture of monomeric and multimeric G-quadruplexes. Appropriate mutation of several bases in the wild sequence produces the shift from parallel to antiparallel G-quadruplex, as well as the disappearance of multimeric species. The shift from the antiparallel to the parallel conformation induced by temperature is reflected in both CD and SEC profiles.

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