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The aim of the presented study was to examine the antimicrobial activity of ethanol extract of Polish propolis (EEPP) against biofilm-forming CoNS strains  in vitro . Our results revealed that EEPP displayed varying degrees of activity against CoNS with MIC values ranging from 1.56 to 0.78 mg/mL. The average MIC was 1.13 ± 0.39 mg/mL while calculated MIC 50  and MIC 90  values were 0.78 mg/mL and 1.56 mg/mL, respectively. The biofilm formation ability by all tested  S. epidermidis  strains was inhibited at EEPP concentrations ranging from 0.39 to 1.56 mg/mL. The degree of reduction of AlamarBlue was directly associated with the proliferation of  S. epidermidis  strains. The increased proliferation of  S. epidermidis  strains was observed after 12 and 24 hours of incubation in the presence of EEPP concentrations ranging from 0.025 to 0.39 mg/mL. These results suggest that antimicrobial activities of EEPP against  S. epidermidis  expressed as the reduction of bacterial growth, reduction of biofilm formation ability, and the intensity of proliferation were significantly affected by incubation time and EEPP concentration used as well as the interactions between these factors.

In VitroAntimicrobial Activity of Ethanolic Extract of Polish Propolis against Biofilm FormingStaphylococcus epidermidisStrains