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The retrogradation rate of waxy rice starch gel was investigated during storage at temperatures in the vicinity of the glass transition temperature of a maximally concentrated system ( T    g  ′), as it was hypothesized that such temperatures might cause different effects on retrogradation. The  T    g  ′ value of fully gelatinized waxy rice starch gel with 50% water content and the enthalpy of melting retrograded amylopectin in the gels were investigated using differential scanning calorimetry. Starch gels were frozen to −30°C and stored at 4, 0, −3, −5, and −8°C for 5 days. The results indicated that the  T    g  ′ value of gelatinized starch gel annealed at −7°C for 15 min was −3.5°C. Waxy rice starch gels retrograded significantly when stored at 4°C with a decrease in the enthalpy of melting retrograded starch in samples stored for 5 days at −3, −5, and −8°C, respectively, perhaps due to the more rigid glass matrix and less molecular mobility facilitating starch chain recrystallization at temperatures below  T    g  ′. This suggests that retardation of retrogradation of waxy rice starch gel can be achieved at temperature below  T    g  ′.

Retrogradation of Waxy Rice Starch Gel in the Vicinity of the Glass Transition Temperature