Optimization of Synthesis of Seleno‐Sargassum fusiforme (Harv.) Setch. Polysaccharide by Response Surface Methodology, Its Characterization, and Antioxidant Activity

The response surface methodology was employed to optimize the synthesis conditions of seleno-Sargassum fusiforme (Harv.) Setch. polysaccharide. Three independent variables (reaction time, reaction temperature, and ratio of Na2SeO3 to SFPSI) were tested. Furthermore, the characterization and antioxidant activity of Se-SFPSI in vivo were investigated. The result showed that the actual experimental Se content of Se-SFPSI was 3.352 mg/g at the optimum reaction conditions of reaction time 8 h, reaction temperature 71°C, and ratio of Na2SeO3 to SFPSIB 1.0 g/g. A series of experiments showed that the characterization of Se-SFPSIB was significantly different from that of SFPSIB. Additionally, antioxidant activity assay indicated that the Se-SFPSIB could increase catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activity of mice bearing tumor S180 in blood, heart, and liver while decreasing malondialdehyde (MDA) levels. It can be concluded that selenylation is a feasible approach to obtain seleno-polysaccharide which was utilized as highly biological medicine or functional food