Neurite Outgrowth in PC12 Cells Stimulated by Components fromDendranthema×grandiflorumcv. “Mottenohoka” Is Enhanced by Suppressing Phosphorylation of p38MAPK | Zendy

Atsuyoshi Nishina | Zendy; Hirokazu Kimura | Zendy; Hiroyuki Tsukagoshi | Zendy; Kunihisa Kozawa | Zendy; Mamoru Koketsu | Zendy; Masayuki Ninomiya | Zendy; Shoei Furukawa | Zendy

Open Access

Neurite Outgrowth in PC12 Cells Stimulated by Components fromDendranthema×grandiflorumcv. “Mottenohoka” Is Enhanced by Suppressing Phosphorylation of p38MAPK

Author(s) -

Atsuyoshi Nishina,

Hirokazu Kimura,

Hiroyuki Tsukagoshi,

Kunihisa Kozawa,

Mamoru Koketsu,

Masayuki Ninomiya,

Shoei Furukawa

Publication year - 2013

Publication title -

evidence-based complementary and alternative medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.552

H-Index - 90

eISSN - 1741-4288

pISSN - 1741-427X

DOI - 10.1155/2013/403503

Subject(s) - neurite , acacetin , luteolin , phosphorylation , mapk/erk pathway , microbiology and biotechnology , chemistry , stimulation , kinase , extracellular , in vitro , biology , biochemistry , flavonoid , neuroscience , apigenin , antioxidant

Components from Dendranthema × grandiflorum cv. “Mottenohoka” that promote neurite outgrowth of PC12 cells were identified and the mechanism of neurite outgrowth stimulated by isolated components was studied. Components that promoted the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK 1/2) of PC12 cells were isolated. From various structural analyses, the active components were identified as acacetin and luteolin. The effects of acacetin or luteolin on PC12 cells were evaluated by electro-blotting and immunostaining. Slight neurite outgrowth in PC12 cells was observed within 2 days of culture after stimulation by luteolin or acacetin. However, NGF-stimulation induced remarkable neurite outgrowth in comparison. Neurite outgrowth by luteolin or acacetin was significantly enhanced by pretreatment with SB203580 (a p38MAPK inhibitor). The results of this study into the phosphorylation of ERK 1/2 and p38MAPK by flavonoids suggest that the inhibition of p38MAPK phosphorylation may effectively enhance neurite outgrowth.

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