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The objective of this study is to establish poultry liver injury model induced by (CCl 4 ) and seek effective hepatoprotective herbals for clinical application. Different doses of CCl 4  dissolved in vegetable oil (1 : 1,  V/V ) were injected via pectoral muscle to induce acute liver injury model in chickens. An herbal formula, Longyin decoction, was prepared for hepatoprotection test on chicken acute liver injury models. The pathologic changes of the liver were observed, and the activities of ALT and AST were, respectively, detected to evaluate the hepatoprotective effects of Longyin decoction on chickens. The chicken acute liver injury model was successfully established by injecting CCl 4  via pectoral muscle. The best dose of CCl 4  inducing chicken liver injury was 4.0 mL/kg · BW (body weight). The results of qualitative determination by HPTLC showed that the components of Longyin decoction contained  Gentian, Capillaries, Gardenia,  and  Bupleurum root . In the high-dose Longyin group and the middle-dose Longyin group, the pathological changes of the damaged liver were mitigated and the activities of ALT and AST in serum were reduced significantly. Longyin decoction has obvious hepatoprotective effect on acute liver injury induced by CCl 4 .

Hepatoprotective Effects of a Chinese Herbal Formula, Longyin Decoction, on Carbon-Tetrachloride-Induced Liver Injury in Chickens