Open AccessAntiproliferative Effect and the Isolated Compounds ofPouzolzia indica
Author(s) -
Chanyapat Sangsuwon,
Weena Jiratchariyakul,
Yaowalak Upratya,
Tanawan Kummalue
Publication year - 2013
Publication title -
evidence-based complementary and alternative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.552
H-Index - 90
eISSN - 1741-4288
pISSN - 1741-427X
DOI - 10.1155/2013/342352
Subject(s) - scopoletin , coumarin , friedelin , chemistry , fractionation , vitaceae , chromatography , glucoside , chemical structure , bioassay , umbelliferone , stereochemistry , organic chemistry , botany , biology , triterpenoid , medicine , alternative medicine , genetics , pathology , vitis vinifera
Previous report showed the high potent antiproliferative effect of the methanolic part extracted from the aerial parts of Pouzolzia indica on NB4 and HT93A acute leukemic cell lines with the IC 50 values of 28.5 and 49.8 μ g/mL, respectively. The bioassay-guided fractionation of the methanolic part gave 5 fractions, that is, FFI–FFV. FFII, FFIII, and FFIV inhibited the above leukemic cell lines with the IC 50 values of 15.1 (FFII), 14.4 (FFIII), 32.1 (FFIV), and 31.0 (FFII), 9.7 (FFIII), 10.5 (FFIV) μ g/mL, respectively. The compounds in these fractions were isolated using chromatographic technique. FFII contained friedelin 1 , 28-hydroxy-3-friedelanone 2 , and 7-methoxy-coumarin 3 . FFIII contained 6, 7-dimethoxy-coumarin 4 , scopoletin 5 , methyl caffeate 6 . FFIV contained sitosteryl glucoside 7 and a supposed glycosphingolipid 8 . The chemical structures were elucidated by spectroscopic methods.
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