Activation of MITF by Argan Oil Leads to the Inhibition of the Tyrosinase and Dopachrome Tautomerase Expressions in B16 Murine Melanoma Cells | Zendy

Myra O. Villareal | Zendy; Sayuri Kume | Zendy; Thouria Bourhim | Zendy; Fatima Zahra Bakhtaoui | Zendy; Kenichi Kashiwagi | Zendy; Junkyu Han | Zendy; Chemseddoha Gadhi | Zendy; Hiroko Isoda | Zendy

Open Access

Activation of MITF by Argan Oil Leads to the Inhibition of the Tyrosinase and Dopachrome Tautomerase Expressions in B16 Murine Melanoma Cells

Author(s) -

Myra O. Villareal,

Sayuri Kume,

Thouria Bourhim,

Fatima Zahra Bakhtaoui,

Kenichi Kashiwagi,

Junkyu Han,

Chemseddoha Gadhi,

Hiroko Isoda

Publication year - 2013

Publication title -

evidence-based complementary and alternative medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.552

H-Index - 90

eISSN - 1741-4288

pISSN - 1741-427X

DOI - 10.1155/2013/340107

Subject(s) - microphthalmia associated transcription factor , tyrosinase , melanin , melanoma , western blot , chemistry , downregulation and upregulation , skin hyperpigmentation , hyperpigmentation , biochemistry , cancer research , biology , enzyme , gene

Argan ( Argania spinosa L.) oil has been used for centuries in Morocco as cosmetic oil to maintain a fair complexion and to cure skin pimples and chicken pox pustules scars. Although it is popular, the scientific basis for its effect on the skin has not yet been established. Here, the melanogenesis regulatory effect of argan oil was evaluated using B16 murine melanoma cells. Results of melanin assay using B16 cells treated with different concentrations of argan oil showed a dose-dependent decrease in melanin content. Western blot results showed that the expression levels of tyrosinase (TYR), tyrosinase-related protein 1 (TRP1), and dopachrome tautomerase (DCT) proteins were decreased. In addition, there was an increase in the activation of MITF and ERK1/2. Real-time PCR results revealed a downregulation of Tyr , Trp1 , Dct , and Mitf mRNA expressions. Argan oil treatment causes MITF phosphorylation which subsequently inhibited the transcription of melanogenic enzymes, TYR and DCT. The inhibitory effect of argan oil on melanin biosynthesis may be attributed to tocopherols as well as the synergistic effect of its components. The results of this study provide the scientific basis for the traditionally established benefits of argan oil and present its therapeutic potential against hyperpigmentation disorders.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research