Short-Term Stability of Biomarkers of Oxidative Stress and Antioxidant Status in Human Serum
Author(s) -
Eugène Jansen,
Piet Beekhof,
Johannes W.J.M. Cremers,
Dalė Vieželienė,
Vladimíra Mužáková,
J Skalický
Publication year - 2013
Publication title -
isrn biomarkers
Language(s) - English
Resource type - Journals
ISSN - 2314-6265
DOI - 10.1155/2013/316528
Subject(s) - antioxidant , oxidative stress , chemistry , antioxidant capacity , oxidative phosphorylation , food science , chromatography , biochemistry
The oxidation and antioxidant status of serum are often determined in serum samples which have been frozen for some time. The oxidative stress process is prone to fast alterations in the sample because of the possible instability of the reactants. Here one oxidation assay (ROM) and three antioxidant assays (FRAP, TAS, and BAP) have been tested on their performance and stability at short-time storage. The most commonly used temperatures for storage and handling of serum samples (
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