Open AccessThe Inhibitory Effect of Ellagic Acid on Cell Growth of Ovarian Carcinoma Cells
Author(s) -
YuanChiang Chung,
Li-Cheng Lu,
Ming-Hsiu Tsai,
Yu-Jen Chen,
YiYing Chen,
Shih-Ping Yao,
ChihPing Hsu
Publication year - 2013
Publication title -
evidence-based complementary and alternative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.552
H-Index - 90
eISSN - 1741-4288
pISSN - 1741-427X
DOI - 10.1155/2013/306705
Subject(s) - anoikis , apoptosis , cell cycle , ovarian carcinoma , cancer research , cell growth , ellagic acid , cell , cell culture , autophagy , doxorubicin , chemistry , biology , ovarian cancer , programmed cell death , microbiology and biotechnology , medicine , chemotherapy , cancer , biochemistry , antioxidant , polyphenol , genetics
Ellagic acid (EA) is able to inhibit the growth of several cancer cells; however, its effect on human ovarian carcinoma cells has not yet been investigated. Ovarian carcinoma ES-2 and PA-1 cells were treated with EA (10 ~ 100 μ M) and assessed for viability, cell cycle, apoptosis, anoikis, autophagy, and chemosensitivity to doxorubicin and their molecular mechanisms. EA inhibited cell proliferation in a dose- and time-dependent manner by arresting both cell lines at the G1 phase of the cell cycle, which were from elevating p53 and Cip1/p21 and decreasing cyclin D1 and E levels. EA also induced caspase-3-mediated apoptosis by increasing the Bax : Bcl-2 ratio and restored anoikis in both cell lines. The enhancement of apoptosis and/or inhibition of autophagy in these cells by EA assisted the chemotherapy efficacy. The results indicated that EA is a potential novel chemoprevention and treatment assistant agent for human ovarian carcinoma.
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