English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Triatoma brasiliensis sensu lato  (s.l.), the main vector of Chagas disease in northeastern Brazil, is a species complex comprising four species, one with two subspecies ( T. brasiliensis brasiliensis ,  T. brasiliensis macromelasoma ,  T. juazeirensis ,  T. sherlocki , and  T. melanica ), and each taxon displaying distinct ecological requirements. In order to evaluate the genetic relationships among nine  T. brasiliensis  s.l. populations from northeastern Brazil, we analyzed their mitochondrial cytochrome c oxidase subunit 1 sequences and suggested a PCR-RFLP assay to distinguish between  T. b. macromelasoma  and  T. b. brasiliensis  subspecies. All the specimens were morphologically identified as  T. b. brasiliensis . The resulting phylogenies identified two major clades that are congruent with the geographical populations studied. Based on collection sites and in accordance with type-location, one clade was identified as the subspecies  T. b. macromelasoma.  The second clade grouped  T. b. brasiliensis  populations. Restriction endonuclease sites were observed in the sequences and used in PCR-RFLP assays, producing distinct fingerprints for  T. b. macromelasoma  and  T. b. brasiliensis  populations. The results suggest that these are different species and that gene flow occurs only among  T. b. brasiliensis  populations, possibly associated with human activity in the area.

journal of tropical medicine

Mitochondrial PCR-RFLP Assay to Distinguish<i>Triatoma brasiliensis macromelasoma</i>from<i>Triatoma brasiliensis brasiliensis</i>Subspecies (Hemiptera: Reduviidae)

Mitochondrial PCR-RFLP Assay to DistinguishTriatoma brasiliensis macromelasomafromTriatoma brasiliensis brasiliensisSubspecies (Hemiptera: Reduviidae)