Open AccessProfiling of Phytochemicals in Tissues from Sclerocarya birrea by HPLC-MS and Their Link with Antioxidant Activity
Author(s) -
Daniela Russo,
Owen Kenny,
Thomas J. Smyth,
Luigi Milella,
Mohammad B. Hossain,
Moussoukhoye Sissokho Diop,
K. Dilip,
Nigel P. Brunton
Publication year - 2013
Publication title -
isrn chromatography
Language(s) - English
Resource type - Journals
ISSN - 2090-8636
DOI - 10.1155/2013/283462
Subject(s) - bark (sound) , flavonoid , chemistry , glycoside , high performance liquid chromatography , antioxidant , proanthocyanidin , traditional medicine , chromatography , tandem mass spectrometry , polyphenol , mass spectrometry , biology , biochemistry , organic chemistry , medicine , ecology
High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed to investigate the differences in phytochemicals in roots, bark, and leaf of Sclerocarya birrea (marula) for methanol and water extracts that exhibited the best antioxidant activities. As many as 36 compounds were observed in the extracts of these tissues of which 27 phenolic compounds were tentatively identified. The HPLC-MS/MS results showed flavonoid glycosides were prominent in leaf extracts while the galloylated tannins were largely in bark and root extracts. Four flavonoid glycosides that were reported for the first time in the marula leaf have been identified. The HPLC-MS/MS studies also illustrated different degrees (highest degree = 3) of oligomerisation and galloylation of tannins in the bark and root extracts.
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