Diversity of Mercury Resistant Escherichia coli Strains Isolated from Aquatic Systems in Rio de Janeiro, Brazil | Zendy

Raquel Costa de Luca Rebello | Zendy; Karen Machado Gomes | Zendy; Rafael Silva Duarte | Zendy; Caio Tavora Coelho da Costa Rachid | Zendy; Alexandre Soares Rosado | Zendy; Adriana Hamond ReguaMangia | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Diversity of Mercury Resistant Escherichia coli Strains Isolated from Aquatic Systems in Rio de Janeiro, Brazil

Author(s) -

Raquel Costa de Luca Rebello,

Karen Machado Gomes,

Rafael Silva Duarte,

Caio Tavora Coelho da Costa Rachid,

Alexandre Soares Rosado,

Adriana Hamond ReguaMangia

Publication year - 2013

Publication title -

international journal of biodiversity

Language(s) - English

Resource type - Journals

eISSN - 2314-4157

pISSN - 2314-4149

DOI - 10.1155/2013/265356

Subject(s) - escherichia coli , rapd , biology , mercury (programming language) , genetic diversity , temperature gradient gel electrophoresis , typing , microbiology and biotechnology , genetics , gene , 16s ribosomal rna , population , demography , sociology , computer science , programming language

Escherichia coli may harbor genetic mercury resistance markers which makes this bacterial species a promising alternative for bioremediation processes. The objective of this study was to investigate phenotypic and genetic characteristics related to diversity and mercury resistance among 178 Escherichia coli strains isolated from residential, industrial, agricultural, and hospital wastewaters and recreational waters at Rio de Janeiro city. Genetic and conventional methods were carried out in order to determine mercury resistance. Random amplification of polymorphic DNA (RAPD-PCR) and denaturing gradient gel electrophoresis (DGGE) were used to investigate genetic variability. RAPD data revealed a high degree of polymorphism among E. coli mercury resistant strains and showed reproducibility and good discriminative results. DGGE typing detected diversity within the merA gene fragment. Our findings represent an improvement in epidemiological studies of HgR E. coli and support the evidence of nonclonal nature of mercury resistant E. coli strains circulating in rural and urban aquatic systems in Rio de Janeiro city

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research