The Effects of Brazilian Green Propolis against Excessive Light-Induced Cell Damage in Retina and Fibroblast Cells | Zendy

Hiromi Murase | Zendy; Masamitsu Shimazawa | Zendy; Mamoru Kakino | Zendy; Kenji Ichihara | Zendy; Kazuhiro Tsuruma | Zendy; Hideaki Hara | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

The Effects of Brazilian Green Propolis against Excessive Light-Induced Cell Damage in Retina and Fibroblast Cells

Author(s) -

Hiromi Murase,

Masamitsu Shimazawa,

Mamoru Kakino,

Kenji Ichihara,

Kazuhiro Tsuruma,

Hideaki Hara

Publication year - 2013

Publication title -

evidence-based complementary and alternative medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.552

H-Index - 90

eISSN - 1741-4288

pISSN - 1741-427X

DOI - 10.1155/2013/238279

Subject(s) - propolis , viability assay , propidium iodide , fibroblast , reactive oxygen species , mapk/erk pathway , chemistry , extracellular , cell damage , microbiology and biotechnology , cell , biology , apoptosis , kinase , biochemistry , programmed cell death , in vitro , food science

Background . We investigated the effects of Brazilian green propolis and its constituents against white light- or UVA-induced cell damage in mouse retinal cone-cell line 661W or human skin-derived fibroblast cells (NB1-RGB). Methods . Cell damage was induced by 3,000lx white light for 24 h or 4/10 J/cm 2 UVA exposure. Cell viability was assessed by Hoechst33342 and propidium iodide staining or by tetrazolium salt (WST-8) cell viability assay. The radical scavenging activity of propolis induced by UVA irradiation in NB1-RGB cells was measured using a reactive-oxygen-species- (ROS-) sensitive probe CM-H 2 DCFDA. Moreover, the effects of propolis on the UVA-induced activation of p38 and extracellular signal-regulated kinase (ERK) were examined by immunoblotting. Results . Treatment with propolis and two dicaffeoylquinic acids significantly inhibited the decrease in cell viability induced by white light in 661W. Propolis and its constituents inhibited the decrease in cell viability induced by UVA in NB1-RGB. Moreover, propolis suppressed the intracellular ROS production by UVA irradiation. Propolis also inhibited the levels of phosphorylated-p38 and ERK by UVA irradiation. Conclusion . Brazilian green propolis may become a major therapeutic candidate for the treatment of AMD and skin damage induced by UV irradiation.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research