Optimization of Aqueous Two-Phase Systems for the Recovery of Soluble Proteins from Tannery Wastewater Using Response Surface Methodology | Zendy

Raja Selvaraj | Zendy; V. Ramachandra Murty | Zendy

Open Access

Optimization of Aqueous Two-Phase Systems for the Recovery of Soluble Proteins from Tannery Wastewater Using Response Surface Methodology

Author(s) -

Raja Selvaraj,

V. Ramachandra Murty

Publication year - 2013

Publication title -

journal of engineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.244

H-Index - 20

eISSN - 2314-4912

pISSN - 2314-4904

DOI - 10.1155/2013/217483

Subject(s) - central composite design , response surface methodology , polyethylene glycol , peg ratio , wastewater , aqueous solution , fractional factorial design , chromatography , factorial experiment , chemistry , aqueous two phase system , sodium citrate , precipitation , sodium , computer science , environmental science , environmental engineering , biochemistry , organic chemistry , medicine , physics , finance , pathology , meteorology , economics , machine learning

Aqueous two-phase system (ATPS) composed of polyethylene glycol 6000 (PEG 6000) and sodium citrate (SC) has been proposed to recover the valuable soluble proteins from tannery wastewater. A sequential optimization strategy which included fractional factorial design (fFD) and central composite design (CCD) was employed to enhance the recovery. From this strategy, a second-order polynomial model was obtained for the protein recovery and it was validated. The optimum recovery was found as 93.46% when pH, NaCl concentration, and temperature were kept at 7.5, 0.1 M, and 33°C, respectively, for a phase system composed of 20% (w/w) PEG 6000-15% (w/w) SC. Thus the proposed ATPS can serve as an alternative to the conventional precipitation method to recover the soluble proteins from tannery wastewater

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research