Peripheral Blood Leukocytes and Serum Nested Polymerase Chain Reaction Are Complementary Methods for Monitoring Active Cytomegalovirus Infection in Transplant Patients | Zendy

P D Andrade | Zendy; MT Fioravanti | Zendy; EBV Anjos | Zendy; Cristiane de Oliveira | Zendy; DM Albuquerque | Zendy; S. G. Costa | Zendy

Open Access

Peripheral Blood Leukocytes and Serum Nested Polymerase Chain Reaction Are Complementary Methods for Monitoring Active Cytomegalovirus Infection in Transplant Patients

Author(s) -

P D Andrade,

MT Fioravanti,

EBV Anjos,

Cristiane de Oliveira,

DM Albuquerque,

S. G. Costa

Publication year - 2013

Publication title -

canadian journal of infectious diseases and medical microbiology

Language(s) - English

Resource type - Journals

eISSN - 1918-1493

pISSN - 1712-9532

DOI - 10.1155/2013/214631

Subject(s) - nested polymerase chain reaction , cytomegalovirus , peripheral blood , polymerase chain reaction , cytomegalovirus infection , medicine , immunology , peripheral , virology , human cytomegalovirus , human immunodeficiency virus (hiv) , herpesviridae , virus , biology , viral disease , biochemistry , gene

Human cytomegalovirus is an important cause of morbidity and mortality in immunocompromised patients. Qualitative polymerase chain reaction (PCR) has proven to be a sensitive and effective technique in defining active cytomegalovirus infection, in addition to having low cost and being a useful test for situations in which there is no need for quantification. Real-time PCR has the advantage of quantification; however, the high cost of this methodology makes it impractical for routine use.

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