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A series of β-Isatin aldehyde-N,N′-thiocarbohydrazone derivatives were synthesized and assayed for their in vitro antimicrobial and antioxidant activity. The new compounds were characterized based on spectral (FT-IR, NMR, MS) analyses. All the test compounds possessed a broad spectrum of activity having MIC values rangeing from 12.5 to 400 μg/ml against the tested microorganisms. Among the compounds 3e, 3j and 3n show highest significant antimicrobial activity. The free radical scavenging effects of the test compounds against stable free radical DPPH (α,α-diphenyl-β-picryl hydrazyl) and H2O2 were measured spectrophotometrically. Compounds 3j, 3n,  3l, and 3e, respectively, had the most effective antioxidant activity against DPPH and H2O2 scavenging activity

Microwave-Assisted Synthesis, Characterization, Antimicrobial and Antioxidant Activity of Some New Isatin Derivatives