Expression Profiling and Proteomic Analysis of JIN Chinese Herbal Formula in Lung Carcinoma H460 Xenografts | Zendy

Luyu Zheng | Zendy; Weiyi Zhang | Zendy; Miao Jiang | Zendy; Huarong Zhang | Zendy; Fei Xiong | Zendy; Yang Yu | Zendy; Meijuan Chen | Zendy; Jing Zhou | Zendy; Xiaoming Dai | Zendy; YuPing Tang | Zendy; Ming Jiang | Zendy; Mingyan Wang | Zendy; Ge Cheng | Zendy; JinAo Duan | Zendy; Wei Yu | Zendy; Biaoyang Lin | Zendy; Haian Fu | Zendy; Xu Zhang | Zendy

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Expression Profiling and Proteomic Analysis of JIN Chinese Herbal Formula in Lung Carcinoma H460 Xenografts

Author(s) -

Luyu Zheng,

Weiyi Zhang,

Miao Jiang,

Huarong Zhang,

Fei Xiong,

Yang Yu,

Meijuan Chen,

Jing Zhou,

Xiaoming Dai,

YuPing Tang,

Ming Jiang,

Mingyan Wang,

Ge Cheng,

JinAo Duan,

Wei Yu,

Biaoyang Lin,

Haian Fu,

Xu Zhang

Publication year - 2013

Publication title -

evidence-based complementary and alternative medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.552

H-Index - 90

eISSN - 1741-4288

pISSN - 1741-427X

DOI - 10.1155/2013/160168

Subject(s) - heat shock protein , traditional chinese medicine , wnt signaling pathway , signal transduction , hedgehog signaling pathway , biology , microbiology and biotechnology , cancer research , gene , medicine , biochemistry , pathology , alternative medicine

Many traditional Chinese medicine (TCM) formulae have been used in cancer therapy. The JIN formula is an ancient herbal formula recorded in the classic TCM book Jin Kui Yao Lue (Golden Chamber). The JIN formula significantly delayed the growth of subcutaneous human H460 xenografted tumors in vivo compared with the growth of mock controls. Gene array analysis of signal transduction in cancer showed that the JIN formula acted on multiple targets such as the mitogen-activated protein kinase, hedgehog, and Wnt signaling pathways. The coformula treatment of JIN and diamminedichloroplatinum (DDP) affected the stress/heat shock pathway. Proteomic analysis showed 36 and 84 differentially expressed proteins between the mock and DDP groups and between the mock and JIN groups, respectively. GoMiner analysis revealed that the differentially expressed proteins between the JIN and mock groups were enriched during cellular metabolic processes, and so forth. The ones between the DDP and mock groups were enriched during protein-DNA complex assembly, and so forth. Most downregulated proteins in the JIN group were heat shock proteins (HSPs) such as HSP90AA1 and HSPA1B, which could be used as markers to monitor responses to the JIN formula therapy. The mechanism of action of the JIN formula on HSP proteins warrants further investigation.

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