Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic ArchaeonPyrococcus furiosus | Zendy

Simona Lobasso | Zendy; Patrizia Lopalco | Zendy; Roberto Angelini | Zendy; Rita Vitale | Zendy; Harald Huber | Zendy; Volker Müller | Zendy; Angela Corcelli | Zendy

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Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic ArchaeonPyrococcus furiosus

Author(s) -

Simona Lobasso,

Patrizia Lopalco,

Roberto Angelini,

Rita Vitale,

Harald Huber,

Volker Müller,

Angela Corcelli

Publication year - 2012

Publication title -

archaea

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.8

H-Index - 40

eISSN - 1472-3654

pISSN - 1472-3646

DOI - 10.1155/2012/957852

Subject(s) - pyrococcus furiosus , lipidome , chemistry , phosphatidylglycerol , archaea , haloarchaea , cardiolipin , lipidomics , biochemistry , cardiolipins , chromatography , phospholipid , phosphatidylcholine , membrane , gene

The lipidome of the marine hyperthermophilic archaeon Pyrococcus furiosus was studied by means of combined thin-layer chromatography and MALDI-TOF/MS analyses of the total lipid extract. 80–90% of the major polar lipids were represented by archaeol lipids (diethers) and the remaining part by caldarchaeol lipids (tetraethers). The direct analysis of lipids on chromatography plate showed the presence of the diphytanylglycerol analogues of phosphatidylinositol and phosphatidylglycerol, the N -acetylglucosamine-diphytanylglycerol phosphate plus some caldarchaeol lipids different from those previously described. In addition, evidence for the presence of the dimeric ether lipid cardiolipin is reported, suggesting that cardiolipins are ubiquitous in archaea.

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