Supercritical SC-CO2and Soxhletn-Hexane Extract of TunisianOpuntia ficus indicaSeeds and Fatty Acids Analysis

The fatty acids profiles of Tunisian Opuntia ficus indica seeds (spiny and thornless form) were investigated. Results of supercritical carbon dioxide (SC-CO 2 ) and soxhlet n -hexane extract were compared. Quantitatively, the better yield was obtained through soxhlet n -hexane: 10.32% (spiny) and 8.91% (thornless) against 3.4% (spiny) and 1.94% (thornless) by SC-CO 2 extract ( T  = 40°C, P = 180 bar, time = 135 mn, CO 2 flow rate = 15 mL · s −1 ). Qualitatively, the main fatty acids components were the same for the two types of extraction. Linoleic acid was the major compound, SC-CO 2 : 57.60% (spiny), 59.98% (thornless), soxhlet n -hexane: 57.54% (spiny), 60.66% (thornless), followed by oleic acid, SC-CO 2 : 22.31% (spiny), 22.40% (thornless), soxhlet n -hexane: 25.28% (spiny), 20.58% (thornless) and palmitic acid, SC-CO 2 : 14.3% (spiny), 12.92% (thornless), soxhlet n -hexane: 11.33% (spiny), 13.08% (thornless). The SC-CO 2 profiles fatty acids showed a richness with other minority compounds such as C 20:1 , C 20:2 , and C 22 .The seeds oil was highly unsaturated (US = 4.44–5.25), and the rising temperatures donot affect the selectivity of fatty acids extract by SC-CO2: US = 4.44 ( T  = 40°C) and 4.13 ( T  = 70°C).