Open AccessTirapazamine-Doxorubicin Interaction Referring to Heart Oxidative Stress and Ca2+Balance Protein Levels
Author(s) -
J Sliwińska,
Jarosław Dudka,
Agnieszka Korga-Plewko,
Franciszek Burdan,
W Matysiak,
Barbara JodłowskaJędrych,
Sławomir Mańdziuk,
K. Dawidek-Pietryka
Publication year - 2012
Publication title -
oxidative medicine and cellular longevity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.494
H-Index - 93
eISSN - 1942-0900
pISSN - 1942-0994
DOI - 10.1155/2012/890826
Subject(s) - tirapazamine , doxorubicin , oxidative stress , chemistry , pharmacology , glutathione , lipid peroxidation , cardiotoxicity , contractility , oxidative phosphorylation , endocrinology , medicine , biochemistry , toxicity , chemotherapy , in vitro , enzyme , cytotoxicity , organic chemistry
Doxorubicin (DOX) causes long-term cardiomyopathy that is dependent on oxidative stress and contractility disorders. Tirapazamine (TP), an experimental adjuvant drug, passes the same red-ox transformation as DOX. The aim of the study was to evaluate an effect of tirapazamine on oxidative stress, contractile protein level, and cardiomyocyte necrosis in rats administered doxorubicin. Rats were intraperitoneally injected six times once a week with tirapazamine in two doses, 5 (5TP) and 10 mg/kg (10TP), while doxorubicin was administered in dose 1.8 mg/kg (DOX). Subsequent two groups received both drugs simultaneously (5TP+DOX and 10TP+DOX). Tirapazamine reduced heart lipid peroxidation and normalised RyR2 protein level altered by doxorubicin. There were no significant changes in GSH/GSSG ratio, total glutathione, cTnI, AST, and SERCA2 level between DOX and TP+DOX groups. Cardiomyocyte necrosis was observed in groups 10TP and 10TP+DOX
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