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We submitted to ozone treatment  Triticum aestivum  (common wheat) seeds severely contaminated by fungi. Fungi colonies developed when seeds were placed over malt agar medium in Petri dishes;  Fusarium sp . and  Alternaria sp . were identified. However, conventional colonies counting did not allow a clear assessment of the effect of ozone disinfection. We thus used self-organizing maps (SOMs) to perform an image analysis of colonies surface area that clearly showed a significant disinfection effect on  Fusarium sp .

Use of Self-Organizing Map to Analyze Images of Fungi Colonies Grown fromTriticum aestivumSeeds Disinfected by Ozone Treatment