A Validated RP-HPLC Method for the Estimation of Pizotifen in Pharmaceutical Dosage Form | Zendy

M. V. Basaveswara Rao | Zendy; A. V. D. Nagendrakumar | Zendy; Sushanta Maiti | Zendy; N. Chandrasekhar | Zendy

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A Validated RP-HPLC Method for the Estimation of Pizotifen in Pharmaceutical Dosage Form

Author(s) -

M. V. Basaveswara Rao,

A. V. D. Nagendrakumar,

Sushanta Maiti,

N. Chandrasekhar

Publication year - 2011

Publication title -

chromatography research international

Language(s) - English

Resource type - Journals

eISSN - 2090-3510

pISSN - 2090-3502

DOI - 10.1155/2012/846574

Subject(s) - chromatography , dosage form , acetonitrile , elution , high performance liquid chromatography , chemistry , methanol , retention time , gradient elution , analytical chemistry (journal) , organic chemistry

A simple, selective, linear, precise, and accurate RP-HPLC method was developed and validated for rapid assay of Pizotifen in pharmaceutical dosage form. Isocratic elution at a flow rate of 1.0 mL/min was employed on Chromosil C18 (250 mm × 4.6 mm, 5 μm) column at ambient temperature. The mobile phase consists of methanol : acetonitrile in the ratio of 10 : 90 v/v. The UV detection wavelength was 230 nm, and 20 μL sample was injected. The retention time for Pizotifen was 2.019 min. The percent RSD for accuracy of the method was found to be 0.2603%. The method was validated as per the ICH guidelines. The method can be successfully applied for routine analysis of Pizotifen in the rapid and reliable determination of Pizotifen in pharmaceutical dosage form

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